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R&D Systems, Inc.

2 ARTICLES PUBLISHED IN JoVE

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Profiling Changes in Receptor Tyrosine Kinase Phosphorylation using Antibody Arrays - ADVERTISEMENT
Greta J. Wegner 1, David J. Finkel 1, Amy E. James 1, Richard A. Krzyzek 1
1Array Group, Assay Department, R&D Systems, Inc.

Proteome Profiler antibody arrays are a convenient and cost efficient way to screen for changes in receptor tyrosine kinase (RTK) phosphorylation without performing numerous immunoprecipitation (IP) Westerns. The ARY001 Human RTK array allows for the qualitative measurement of multiple RTKs in a single sample using chemiluminescence detection.

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Biology

Induction and Analysis of Epithelial to Mesenchymal Transition
Yixin Tang 1, Greg Herr 2, Wade Johnson 2, Ernesto Resnik 1, Joy Aho 2
1Antibody Development Department, R&D Systems, Inc., 2Stem Cells and Developmental Biology Department, R&D Systems, Inc.

A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.

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