JoVE Logo

Sign In

Radboud University Medical Center, Nijmegen, The Netherlands

1 ARTICLES PUBLISHED IN JoVE

image

Genetics

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Jos G.A. Smits *1, Jieqiong Qu *1, Hanna Niehues 2, Huiqing Zhou 1,3
1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands

Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved