JoVE Logo

Sign In

Royal Institute of Technology (KTH)

2 ARTICLES PUBLISHED IN JoVE

image

Environment

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
Colin Ruprecht 1, Takayuki Tohge 1, Alisdair Fernie 1, Cara L. Mortimer 2, Amanda Kozlo 2, Paul D. Fraser 2, Norma Funke 1, Igor Cesarino 3,4, Ruben Vanholme 3,4, Wout Boerjan 3,4, Kris Morreel 3,4, Ingo Burgert 5,6, Notburga Gierlinger 5,6, Vincent Bulone 7, Vera Schneider 8, Andrea Stockero 8, Juan Navarro-Aviñó 9, Frank Pudel 10, Bart Tambuyser 11, James Hygate 12, Jon Bumstead 13, Louis Notley 13, Staffan Persson 1,14
1Max Planck Institute for Molecular Plant Physiology, 2School of Biological Sciences, Plant Molecular Science, Centre for Systems and Synthetic Biology, Royal Holloway, University of London, 3Department of Plant Systems Biology, VIB, 4Department of Plant Biotechnology and Bioinformatics, UGhent, 5Institute for Building Materials, ETH Zurich, 6Applied Wood Materials, EMPA, 7Division of Glycoscience, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH), 8European Research and Project Office GmbH, 9ABBA Gaia S.L., 10Pflanzenöltechnologie, 11Capax Environmental Services, 12Green Fuels, 13Neutral Consulting Ltd, 14Plant Cell Biology Research Centre, School of Botany, University of Melbourne

Plant biomass offers a renewable resource for multiple products, including fuel, feed, food, and a variety of materials. In this paper we investigate the properties of tobacco tree (Nicotiana glauca) and poplar as suitable sources for a biorefinery pipeline.

image

Biology

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
M. Angélica Bello 1, Yolanda Ruiz-León 2, J. Vladimir Sandoval-Sierra 3, Svetlana Rezinciuc 4, Javier Diéguez-Uribeondo 3
1Biodiversity and Conservation Department, Real Jardín Botánico, CSIC, 2Research Support Unit, Real Jardín Botánico, CSIC, 3Mycology Department, Real Jardín Botánico, CSIC, 4Division of Glycoscience, AlbaNova University Center, Royal Institute of Technology (KTH)

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved