Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage AssayIntisar Koch 1, Matthew J. Schellenberg 2, Francisco Bustos 1,3
1Pediatrics and Rare Diseases Group, Sanford Research, 2Department of Biochemistry and Molecular Biology, Mayo Clinic, 3Department of Pediatrics, Sanford School of Medicine, University of South Dakota
This protocol explains how to measure the effect of genetic variation associated with neurodevelopmental disorders on deubiquitylating enzyme activity by combining recombinant protein purification with ubiquitin chain cleavage assays.
