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Biology

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Heike Summer 1, René Grämer 2, Peter Dröge 1
1School of Biological Sciences, Nanyang Technological University, Singapore - NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)

Denaturing urea polyacrylamide gel electrophoresis is used to separate single-stranded DNA or RNA up to a limit of 500 nucleotides. Urea in combination with heat denatures samples and unstructured single strands migrate within the gel matrix according to their molecular weight.

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