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St. Elizabeth's Medical Center

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
Lama Mulki *1,2, J. Harry Sweigard *1, Kip M. Connor 1
1Angiogenesis Laboratory, Department of Ophthalmology, Harvard Medical School, Massachusetts Eye & Ear Infirmary, 2St. Elizabeth's Medical Center

Here, we present a protocol that allows the investigator to assess leukocyte recruitment dynamics ex vivo by connecting a chamber coated with endothelial-derived adhesion molecules to the circulatory system of a mouse. This method offers significant advantages since it allows for leukocyte assessment under relative biological conditions.

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