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Stazione Zoologica, Anton Dohrn, Naples (Italy)

2 ARTICLES PUBLISHED IN JoVE

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Biology

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana
Loredana Manfra 1, Federica Savorelli 2, Marco Pisapia 1, Erika Magaletti 1, Anna Maria Cicero 1
1Department of Environmental Quality Monitoring, Institute for Environmental Protection and Research, 2Regional Agency for Environmental Protection in Emilia-Romagna

This study concerns the development and standardization of a valuable methodological protocol to determine long-term (14 days) lethal toxicity exerted by chemical substances, industrial wastewater or sewage and liquid environmental samples on the saltwater crustacean, Artemia franciscana.

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Environment

Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants
Alice Rotini 1,2, Loredana Manfra 1,3, Filippo Spanu 2, Marco Pisapia 1, Anna Maria Cicero 1, Luciana Migliore 2
1Institute for Environmental Protection and Research (ISPRA), Rome (Italy), 2Department of Biology, Tor Vergata University, Rome (Italy), 3Department of Biology and Evolution of Marine Organisms, Stazione Zoologica, Anton Dohrn, Naples (Italy)

This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.

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