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Texas A&M School of Dentistry

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
Changchun Dong *1, Bikash Lamichhane *1, Yongxu Zhang 1, Xiaofang Wang 1
1Department of Biomedical Sciences, Texas A&M School of Dentistry

The mouse incisor contains valuable label-retaining cells in its stem cell niche. We have a novel way to unbiasedly detect and quantify the label-retaining cells; our study used EdU labeling and a 3D reconstruction approach after PEGASOS tissue clearing of the mandible.

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