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Texas A&M University-College Station

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Autofluorescence Imaging to Evaluate Cellular Metabolism
Anna Theodossiou 1, Linghao Hu 1, Nianchao Wang 1, Uyen Nguyen 1, Alex J. Walsh 1
1Department of Biomedical Engineering, Texas A&M University-College Station

This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.

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