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Touro University California

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
Sophia Dao 1, Kenton Jones 1, Tamira Elul 1
1College of Osteopathic Medicine, Touro University California

This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.

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