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Uluova Dairy Farm

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release
Berfin Sucu 1, Ayşe Bayraktar 1,2, Hatice Duman 1, Ayşenur Arslan 1, Merve Kaplan 1, Melda Karyelioğlu 1, Eda Ntelitze 1, Taner Taştekin 1, Seray Yetkin 1, Melih Ertürk 2, Steven A. Frese 3,4, Bethany M. Henrick 4,5, Hacı Mehmet Kayili 6, Bekir Salih 7, Sercan Karav 1
1Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, 2Uluova Dairy Farm, 3Department of Nutrition, University of Nevada, 4Department of Food Science and Technology, University of Nebraska, 5Evolve BioSystems Inc., 6Department of Biomedical Engineering, Karabuk University, 7Department of Chemistry, Hacettepe University

Bifidobacteria possess a unique genomic capability for N-glycan cleavage. Recombinantly producing these enzymes would be a promising novel tool to release bioactive N-glycans from glycoprotein-rich substrates such as colostrum.

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Biochemistry

Determining Total Protein and Bioactive Protein Concentrations in Bovine Colostrum
Ayşenur Arslan 1, Hatice Duman 1, Merve Kaplan 1, Hasan Uzkuç 1, Ayşe Bayraktar 1,2, Melih Ertürk 2, Merve Alkan 1,2, Steven A. Frese 3,4, Rebbeca M. Duar 5, Bethany M. Henrick 4,5, Sercan Karav 1
1Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, 2Uluova Dairy Farm, 3Department of Nutrition, University of Nevada, Reno, 4Department of Food Science and Technology, University of Nebraska, Lincoln, 5Evolve BioSystems Inc.

Bovine colostrum is both a primary source of nutrients and immunological support for the newborn calf. The understanding of the level of therapeutic proteins (lactoferrin and IgG) is important for the bovine colostrum dosing and standardization for human consumption.

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