Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell EnvironmentQuentin Lemaire 1, Marie Duhamel 1, Antonella Raffo-Romero 1, Michel Salzet 1, Christophe Lefebvre 1
1U1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), Univ. Lille, INSERM
The present report highlights chronological requirements for extracellular vesicle (EV) isolation from microglia or blood macrophages. Microglia-derived EVs were evaluated as regulators of the neurite outgrowth while blood macrophage-derived EVs were studied in the control of C6 glioma cell invasion in in vitro assays. The goal is to better understand these EV functions as immune mediators in specific microenvironments.
