University of California, Berkeley and University of California, San Francisco

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Andre Lai^*1, Rachel Rex^*2, Kristen L. Cotner¹, Alan Dong³, Michael Lustig^1,3, Lydia L. Sohn^1,2

¹Graduate Program in Bioengineering, University of California, Berkeley and University of California, San Francisco, ²Department of Mechanical Engineering, University of California, Berkeley, ³Department of Electrical Engineering and Computer Sciences, University of California, Berkeley

Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.