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University of California, Santa Barbara

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
Leah P. Foltz 1, Dennis O. Clegg 1
1Center for Stem Cell Biology and Engineering, University of California, Santa Barbara

This protocol describes how to produce retinal pigment epithelial cells (RPE) from pluripotent stem cells. The method uses a combination of growth factors and small molecules to direct the differentiation of stem cells into immature RPE in fourteen days and mature, functional RPE after three months.

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Neuroscience

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae
Jiangqu Liu *1, Takaaki Sokabe *2,3, Craig Montell 1
1Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, 2Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 3Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences

Here, we present a protocol to determine the preferred environmental temperature of Drosophila larvae using a continuous thermal gradient.

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Biology

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
Lyndsay L. Leach 1, G. Burch Fisher 2, Jeffrey M. Gross 1,3
1Department of Ophthalmology, Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, 2Earth Research Institute, University of California, Santa Barbara, 3Department of Developmental Biology, University of Pittsburgh School of Medicine

This protocol describes the methodology to genetically ablate the retinal pigment epithelium (RPE) using a transgenic zebrafish model. Adapting the protocol to incorporate signaling pathway modulation using pharmacological compounds is extensively detailed. A MATLAB platform for quantifying RPE regeneration based on pigmentation was developed and is presented and discussed.

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