University of Minnesota, Minneapolis

2 ARTICLES PUBLISHED IN JoVE

Medicine

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Callie L. Janik^1,2, Timothy K. Starr^1,2

¹Department of Obstetrics, Gynecology & Women's Health, Masonic Cancer Center, University of Minnesota, Minneapolis, ²Department of Genetics, Cell Biology & Development, Center for Genome Engineering, University of Minnesota, Minneapolis

A method of identifying unknown drivers of carcinogenesis using an unbiased approach is described. The method uses the Sleeping Beauty transposon as a random mutagen directed to specific tissues. Genomic mapping of transposon insertions that drive tumor formation identifies novel oncogenes and tumor suppressor genes

Bioengineering

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

Allison M. Bock^*1,2, David Knorr^*1,2, Dan S. Kaufman^1,2

¹Department of Medicine (Hematology, Oncology, and Transplant), University of Minnesota, Minneapolis, ²Stem Cell Institute, University of Minnesota, Minneapolis

This protocol describes the development, expansion, and in vivo imaging of NK cells derived from hESCs and iPSCs.