University of Nice - Sophia Antipolis

1 ARTICLES PUBLISHED IN JoVE

Biology

Biochemical Titration of Glycogen In vitro

Joffrey Pelletier¹, Grégory Bellot¹, Jacques Pouysségur¹, Nathalie M. Mazure¹

¹Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 - INSERM, U1081 - UNS, Centre Antoine Lacassagne, University of Nice - Sophia Antipolis

We describe here an accurate, reproducible and convenient biochemical method for titration of glycogen in vitro. This technique uses the Abcam Glycogen assay kit and is based on successive hydrolysis of glycogen to glucose and glucose titration by fluorescence.