Detection of DNA Breaks in Dividing Human Cells by Neutral Comet AssayAnthony Nelligan 1, Huzefa Dungrawala 1
1Department of Molecular Biosciences, University of South Florida, Tampa
This protocol is designed to investigate the extent of DNA damage occurring during DNA replication. Under neutral conditions, the induction of DNA breaks can be readily assessed within a short time frame. Additionally, the protocol is adaptable to other cell types and various replication stress reagents.
