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University of Toledo

4 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Imaging Centrosomes in Fly Testes
Marcus L. Basiri 1, Stephanie Blachon 1, Yiu-Cheung Frederick Chim 1, Tomer Avidor-Reiss 1
1Department of Biological Sciences, University of Toledo

Imaging of centrosomal proteins during Drosophila spermatogenesis is a powerful method to identify new proteins critical for centrosome biology as well as to elucidate the particular function of known players in this process.

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Chemistry

Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO2 Source
Mohit Kapoor 1,2, Pratibha Chand-Thakuri *1,2, Justin M. Maxwell *1,2, Michael C. Young 1,2
1Department of Chemistry and Biochemistry, University of Toledo, 2School of Green Chemistry and Engineering, University of Toledo

Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO2. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups.

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Biochemistry

Reconstitution of Msp1 Extraction Activity with Fully Purified Components
Heidi L. Fresenius 1, Matthew L. Wohlever 1
1Department of Chemistry & Biochemistry, University of Toledo

Here, we present a detailed protocol for reconstitution of Msp1 extraction activity with fully purified components in defined proteoliposomes.

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Bioengineering

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
Qingliang Yang 1, Jordan C. Langston 2, Yuan Tang 3, Balabhaskar Prabhakarpandian 4, Laurie E. Kilpatrick 5, Mohammad F. Kiani 1,2,6
1Department of Mechanical Engineering, Temple University, 2Department of Bioengineering, Temple University, 3Department of Bioengineering, University of Toledo, 4Biomedical Technology, CFD Research Corporation, 5Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine, Temple University, 6Department of Radiation Oncology, Lewis Katz School of Medicine, Temple University

In this protocol, a biomimetic microfluid assay, which can reproduce a physiologically relevant microvascular environment and reproduce the entire leukocyte adhesion/migration cascade, is employed to study leukocyte-endothelial cell interactions in inflammatory disease.

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