JoVE Logo

Sign In

University of Toronto - Mississauga

1 ARTICLES PUBLISHED IN JoVE

image

Developmental Biology

Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization
Urfa Arain 1, Priscilla Valentino 1, Ishrat Maliha Islam 1, Ted Erclik 1
1Departments of Biology and Cell & Systems Biology, University of Toronto - Mississauga

This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures. 

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved