Vidyasirimedhi Institute of Science and Technology (VISTEC)

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Rodjarin Kongkaew¹, Chayasith Uttamapinant¹, Maturada Patchsung¹

¹School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC)

Here, we present the step-by-step preparation of premixed, lyophilized recombinase-based isothermal amplification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based reactions, which can be used for the detection of nucleic acid biomarkers of infectious disease pathogens or other genetic markers of interest.