JoVE Logo

Sign In

Waters Corporation

2 ARTICLES PUBLISHED IN JoVE

image

Basic Principles for Purification Using Supercritical Fluid Chromatography - ADVERTISEMENT
Jo-Ann M. Jablonski 1, Christopher J. Hudalla 1, Kenneth J. Fountain 1, Steven M. Collier 1, Damian Morrison 1
1Chemistry Commercial Operations, Waters Corporation

Basic principles for the separation of compounds from mixtures using supercritical fluid chromatography (SFC) are similar to the fundamentals of preparative liquid chromatography.

image

Chemistry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
Catalin Doneanu *1, Jing Fang *1, Yun Alelyunas *1, Ying Qing Yu 1, Mark Wrona 1, Weibin Chen 1
1Waters Corporation

Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved