Westmead Institute for Medical Research

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Rekha Marimuthu^1,2, Habib Francis^1,2, Suat Dervish³, Stephen C.H. Li⁴, Heather Medbury^*1,2, Helen Williams^*1,2

¹Department of Surgery, Vascular Biology Research Centre, Westmead Hospital, ²Westmead Clinical School, Department of Surgery, The University of Sydney, ³Westmead Research Hub, Westmead Institute for Medical Research, ⁴Institute for Clinical Pathology and Medical Research, Westmead Hospital

Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of the expression of M1 (inflammatory) and M2 markers (anti-inflammatory).