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the Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases

4 ARTICLES PUBLISHED IN JoVE

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Environment

Choice and No-Choice Bioassays to Study the Pupation Preference and Emergence Success of Ectropis grisescens
Cai Wang 1, Huifang Wang 1, Tao Ma 1,2, Qiang Xiao 3, Panrong Cao 4, Xuan Chen 5, Hongpeng Xiong 1, Wenquan Qin 1, Zhaohui Sun 1, Xiujun Wen 1
1Guangdong Key Laboratory for Innovation Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 2College of Agriculture, South China Agricultural University, 3Tea Research Institute, Chinese Academy of Agricultural Sciences, 4College of Horticulture, South China Agricultural University, 5College of Coast and Environment, Louisiana State University

Here, we present a protocol to investigate the pupation preference of mature larvae of Ectropis grisescens in response to soil factors (e.g., substrate type and moisture content) using choice bioassays. We also present a protocol of no-choice bioassays to determine the factors that affect the pupation behaviors and survivorship of E. grisescens.

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Cancer Research

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression
Shuang Chen 1, Andreas Giannakou 1, Jonathon Golas 1, Kenneth G. Geles 1
1Oncology R&D group, Pfizer Worldwide Research and Development

An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor phenotype.

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Medicine

Application of Straight-needle, Three-tailed, Knot-free, Peritoneal Sutures in Laparoscopic Transabdominal Preperitoneal Hernia Repair
Ning Ma *1, Zhen Zong *2, Fu-xing Tang 1, En-min Huang 1, Tao Ma 1, Wei-sheng Yang 1, Jia-lin Chen 1, Shuang Chen 1, Tai-cheng Zhou 1
1Department of Gastroenterological Surgery and Hernia Center, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, 2Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Nanchang University

Here, we present a protocol to introduce a new peritoneal suture method. This method is called straight-needle, three-tailed, knot-free suture, and we will outline the manufacturing method and clinical application of this suture in detail.

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Medicine

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair
Ning Ma *1, Dai Tang *2, Fu-xing Tang 1, En-min Huang 1, Tao Ma 1, Wei-sheng Yang 1, Chuang-xiong Liu 1, Hao-nan Huang 1, Shuang Chen 1, Tai-cheng Zhou 1
1Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhou, Sun Yat-sen University, 2Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi)

Presented here is a method for improving the mesh placement in laparoscopic incisional hernia repair, which can shorten the time required for mesh fixation and reduce the occurrence of postoperative chronic pain.

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