Accedi

Wright-Patterson AFB

3 ARTICLES PUBLISHED IN JoVE

Biology

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Jennifer A. Martin^1,2, Joshua E. Smith^1,2, Mercedes Warren¹, Jorge L. Chávez^1,3, Joshua A. Hagen¹, Nancy Kelley-Loughnane¹

¹711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, ²The Henry M. Jackson Foundation, ³UES, Inc.

A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.

Biology

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Joshua E. Smith¹, Jorge L. Chávez^2,3, Joshua A. Hagen², Nancy Kelley-Loughnane²

¹Department of Science and Mathematics, Alvernia University, ²711^th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force Base, ³UES, Inc.

The design and development of an aptamer–gold nanoparticle colorimetric assay for the detection of small molecules for in-the-field applications was examined. Additionally, a smart-device colorimetric application (app) was validated and long-term storage of the assay was established for use in the field.

Biology

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Irina Drachuk^1,2, Svetlana Harbaugh², Nancy Kelley-Loughnane³, Jorge L. Chávez²

¹UES Inc., ²711th Human Performance Wing, Airman Systems Directorate, Air Force Research Laboratory, Wright-Patterson AFB, ³Materials and Manufacturing Directorate, Wright-Patterson AFB

The protocol describes the formation of robust and biocompatible DNA-laden microcapsules as multiplexed in vitro biosensors capable of tracking several ligands.