Construction of Adenoviral Vectors using DNA Assembly TechnologyXiaohui Zou *1, Yun Zhu *2, Chenglong Li 1,3, Yali Duan 2, Linlin Zhang 2, Xiaojuan Guo 1, Wenzhe Hou 1, Zhengde Xie 2,4, Zhuozhuang Lu 1,5
1NHC Key Laboratory of Medical Virology and Viral Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 2Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, 3Henan Chemical Technician College, 4Research Unit of Critical Infection in Childre, Chinese Academy of Medical Sciences, 5Chinese Academy of Sciences Joint Research Center for Emerging Infectious Diseases and Biosafety, Chinese Center for Disease Control and Prevention-Wuhan Institute of Virology
In this study, an infectious clone of human adenovirus type 7 (HAdV-7) was constructed, and an E3-deleted HAdV-7 vector system was established by modifying the infectious clone. This strategy used here can be generalized to make gene transfer vectors from other wild-type adenoviruses.
