Accedi

Institute of Molecular Biology (IMB)

1 ARTICLES PUBLISHED IN JoVE

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

¹Institute of Molecular Biology (IMB), ²Max Planck Institute for Polymer Research

The present protocol describes a method measuring absolute DNA densities within adherent cell nuclei using Voronoi tessellation of single-molecule localization microscopy data, known volume, genome size, and cell cycle stage.