Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux AnalysisJan Van den Bossche 1, Jeroen Baardman 1, Menno P.J. de Winther 1
1Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam
Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.
