Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell CultureMichael Müller 1, Jana Becher 2, Matthias Schnabelrauch 2, Marcy Zenobi-Wong 1
1Department of Health Science & Technology, Cartilage Engineering & Regeneration, 2Biomaterials Department, Innovent e.V.
A bioprinter was used to create patterned hydrogels based on a sacrificial mold. The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.
