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Oslo University Hospital

6 ARTICLES PUBLISHED IN JoVE

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Biology

Xenotransplantation of Human Stem Cells into the Chicken Embryo
Jean-Luc Boulland 1, Gabor Halasi 1, Nedim Kasumacic 1, Joel C. Glover 1,2
1Department of Physiology, University of Oslo, 2Norwegian Center for Stem Cell Research, University of Oslo

In this paper we present a method for transplanting human stem cells into various regions of the central nervous system of the chicken embryo. This provides an in vivo model for assessing the proliferation and differentiation of various types of human stem cells in embryonic tissue environments.

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Medicine

A Neonatal Mouse Spinal Cord Compression Injury Model
Mark Züchner 1,2, Joel C. Glover 2,3, Jean-Luc Boulland 2,3
1Department of Neurosurgery, Oslo University Hospital, 2Norwegian Center for Stem Cell Research, Oslo University Hospital, 3Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Physiology, Institute of Basic Medical Sciences, University of Oslo

This article describes a method for generating a reproducible spinal cord compression injury (SCI) in the neonatal mouse. The model provides an advantageous platform for studying mechanisms of adaptive plasticity that underlie spontaneous functional recovery.

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Medicine

The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
Ane M. Holme 1,2, Maia B. Holm 1,2, Marie C. P. Roland 1,3, Hildegunn Horne 1,2, Trond M. Michelsen 1,3, Guttorm Haugen 2,4, Tore Henriksen 1,2
1Department of Obstetrics, Oslo University Hospital, 2Institute of Clinical Medicine, University of Oslo, 3Norwegian Advisory Unit on Women's Health, Oslo University Hospital, 4Department of Fetal Medicine, Oslo University Hospital

We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling.

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Neuroscience

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy
Lauritz Hagen Kennedy 1, Johanne Egge Rinholm 1,2
1Division of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 2Department of Microbiology, Oslo University Hospital

Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented.

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Biology

Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion
Hege Brincker Fjerdingstad 1, Joel C. Glover 1,2
1Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, 2Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo

To avoid the limitations associated with the enzymatic or mechanical passaging of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) cultured on feeder cells, we have established a fast, effective, cost-efficient, high-yield method for harvesting hESC or hiPSC colonies maintained on a feeder cell layer of human foreskin fibroblasts using EDTA-mediated dis-adhesion.

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Neuroscience

Application of Flow Cytometric Analysis for Measuring Multiple Mitochondrial Parameters in 3D Brain Organoids
Sharika Marjan 1,2, Tsering Yangzom 1,2, Cecilie Katrin Kristiansen 1,3, Anbin Chen 4, Laurence A. Bindoff 1,5,6, Kristina Xiao Liang 1,3
1Department of Clinical Medicine (K1), University of Bergen, 2Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, 3Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 4Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 5Department of Neurology, Haukeland University Hospital, 6National Advisory Unit for Congenital Metabolic Diseases, Oslo University Hospital

This protocol reports a unique method of using a streaming cytometer and multiple antibodies for simultaneous assessment of multiple mitochondrial functional parameters, including changes in mitochondrial volume, amounts of the mitochondrial respiratory chain (MRC) complex subunits, and mitochondrial DNA (mtDNA) replication.

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