Tsurumi University School of Dental Medicine

2 ARTICLES PUBLISHED IN JoVE

Genetics

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Takatoshi Murata¹, Ayako Okada¹, Khairul Matin^1,2,3, Nobuhiro Hanada¹

¹Department of Translational Research, Tsurumi University School of Dental Medicine, ²Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, ³Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

We describe a facile, rapid, and relatively inexpensive method for the generation of gene-disrupted Streptococcus mutans strains; this technique may be adapted for the generation of gene-disrupted strains of various species.

Immunology and Infection

Purification of a High Molecular Mass Protein in Streptococcus mutans

Takatoshi Murata¹, Mamiko Yamashita¹, Masao Ishikawa^1,2, Koji Shibuya², Nobuhiro Hanada¹

¹Department of Translational Research, Tsurumi University School of Dental Medicine, ²Laboratory for Oral Health Science

Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.