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University of Montpellier

3 ARTICLES PUBLISHED IN JoVE

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Behavior

Uncovering Beat Deafness: Detecting Rhythm Disorders with Synchronized Finger Tapping and Perceptual Timing Tasks
Simone Dalla Bella 1,2,3,4, Jakub Sowiński 3
1Movement to Health Laboratory (EuroMov), University of Montpellier, 2Institut Universitaire de France, 3Department of Cognitive Psychology, University of Finance and Management in Warsaw, 4International Laboratory for Brain, Music, and Sound Research (BRAMS)

Behavioral tasks that allow for the assessment of perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) are presented. Synchronization of finger tapping to the beat of an auditory stimuli and detecting rhythmic irregularities provides a means of uncovering rhythm disorders.

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Neuroscience

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
Jolanta Jagodzinska *1, Emmanuelle Sarzi *1, Mélanie Cavalier 1, Marie Seveno 1, Volker Baecker 2, Christian Hamel 1,3,4, Marie Péquignot 1, Cecile Delettre 1
1INSERM U1051, Institut of Neurosciences of Montpellier, 2CNRS UMS3426, BioCampus Montepellier, 3University of Montpellier, 4CHRU Montpellier, Centre of Reference for Genetic Sensory Diseases, CHU Gui de Chauliac Hospital

This manuscript describes a protocol for the in vivo imaging of the mouse retina with high-resolution spectral domain optical coherence tomography (SD-OCT). It focuses on retinal ganglion cells (RGC) in the peripapillary region, with several scanning and quantifying approaches described.

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Bioengineering

Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates
Mahnaz Sabeti Azad *1, Angelo Cardoso Batista *1, Jean-Loup Faulon 1, Chase L. Beisel 2,3, Jerome Bonnet 4, Manish Kushwaha 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier

Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of Escherichia coli BL21 Rosetta2 (ΔrecBCD and ΔrecB). This is a fast, easy, and direct approach for expression in cell-free protein synthesis systems using linear DNA templates.

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