University of Ontario Institute of Technology

2 ARTICLES PUBLISHED IN JoVE

Education

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts

¹Faculty of Science, University of Ontario Institute of Technology

The goal of this manuscript is to demonstrate a straightforward method for the preparation of ynones from acyl chloride and potassium alkynyltrifluoroborate salt starting materials. The one-pot reaction proceeds rapidly in the presence of boron trichloride without exclusion of air and moisture.

Biochemistry

Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

Richard V. Augimeri¹, Andrew J. Varley¹, Janice L. Strap¹

¹Molecular Microbial Biochemistry Laboratory, Faculty of Science (Applied Bioscience Program), University of Ontario Institute of Technology

The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.