University of the Sciences in Philadelphia

1 ARTICLES PUBLISHED IN JoVE

Biology

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Alexander Braun¹, Nicole M. Caesar¹, Kyvan Dang¹, Kenneth A. Myers¹

¹Department of Biological Sciences, University of the Sciences in Philadelphia

Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.