Lentiviral Transduction of HEK293/TN-XXL/Gqi5 Cells
2:49
Preparation for FACS and Isolation of the Single CNiFER Clones
5:17
CNiFER Implantation into Mouse Cortex
7:52
In Vivo Imaging of CNiFER Clones
10:42
Results: Examples of Flexstation Analysis, Agonist-induced FRET Response and Dose Response Curves for D2 CNiFER
12:02
Conclusion
Trascrizione
The overall goal of this video is to describe the methodology for the construction and testing of Cell-based Neurotransmitter Fluorescent Reporters, or CNiFERs, which can be used to optically monitor the release of specific neurotransmitters in vi
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We present a protocol to create cell-based neurotransmitter fluorescent engineered reporters (CNiFERs) for the optical detection of volumetric neurotransmitter release.