Visualization and Stimulation of Presynaptic Fibers
8:04
Results: Optogenetic Activation of Long Range and Local Projections
10:02
Conclusion
Trascrizione
The overall goal of this experiment is to investigate functional and synaptic properties of neural projections and micro-circuits using ex-vivo optogenetics. This includes expression of channel rhodopsin and its activation in specific projections
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Optogenetic approaches are widely used to manipulate neural activity and assess the consequences for brain function. Here, a technique is outlined that upon in vivo expression of the optical activator Channelrhodopsin, allows for ex vivo analysis of synaptic properties of specific long range and local neural connections in fear-related circuits.