Results: Representative F-actin Synaptic Structures of Rat Cortical Neurons
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Conclusion
Trascrizione
The overall goal of this Quantification of Filamentous Actin Punta in cultured neurons, is to introduce a useful method for studying the integrity and cognitivity of synaptic structures. This method can help address key questions in the neuroscien
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Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.