Resolution of Whole Cell Extracts by High Resolution SDS-PAGE
6:20
Analysis of IRF3 Dimerization by Native-PAGE
7:37
Immunoblot Analysis of IRF3 Species
9:23
Results: Detection Distinct Phosphorylated IRF3 Species
10:56
Conclusion
Trascrizione
The overall goal of this procedure is to allow a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. So this method can help answer key questions regarding the interferon mediate innate immune response.
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Here we describe a procedure allowing a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. This is achieved through the combination of a high resolution SDS-PAGE and a native-PAGE coupled to immunoblots using multiple phosphospecific antibodies.