One-dimension Long-length Electrostatic Repulsion-hydrophilic Interaction Chromatography-tandem Mass Spectrometry (LERLIC-MS/MS) Separation and Data Analysis
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Results: Separation of Deamidation Isoforms of Glutamine and Asparagine by LERLIC-MS/MS
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Conclusion
Trascrizione
The overall goal of this procedure is to use shotgun proteomics to characterize and quantify endogenous glutamine and asparagine deamidation products in complex proteomes. This method can help answer key questions in biochemistry, such as the infl
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Here we present a step-by-step protocol of the long-length electrostatic repulsion-hydrophilic interaction chromatography-tandem mass spectrometry (LERLIC-MS/MS) method. This is a novel methodology that enables for the first time quantification and characterization of the glutamine and asparagine deamidation isoforms by shotgun proteomics.