Dissection of Adult Drosophila Anterior Malpighian Tubules
4:32
Validation of Imaging Protocol and Tubule Health
9:47
Results: Quantification of Acid Extrusion in Malpighian Tubule Epithelia
10:56
Conclusion
Trascrizione
The overall goal of this imaging protocol is to describe the quantification of intracellular pH, using a genetically-encoded pH indicator and demonstrate how this method can be used to assess basolateral proton transport in an insect renal structu
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Cellular ion transport can often be assessed by monitoring intracellular pH (pHi). Genetically Encoded pH-Indicators (GEpHIs)Â provide optical quantification of intracellular pH in intact cells. This protocol details the quantification of intracellular pH through cellular ex vivo live-imaging of Malpighian tubules of Drosophila melanogaster with pHerry, a pseudo-ratiometric ge