Deprotection and Purification of RNA Oligonucleotides
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Characterization via MALDI-TOF and RNA Structure Analysis vi CD
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Results: Characterization of RNA Oligomers Containing 2'-Othiophenylmethyl Modification
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Conclusion
Trascrizione
The overall goal of this report is to illustrate the procedure to obtain oligonucleotides of RNA via solid-phase synthesis. Dodecamers of RNA will be synthesized as models and their purification and characterization will also be demonstrated. Thro
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This article provides a detailed procedure on the solid-phase synthesis, purification, and characterization of dodecamers of RNA modified at the C2'-O-position. UV-vis and circular dichroism photometric analyses are used to quantify and characterize structural aspects, i.e., single-strands or double-strands.