Purification of Naïve Human CD4+ Cells, Polarization to Th1 and TH2 Cells, and Nuclei Extraction
3:18
Transposition Reaction and Library Amplification
4:33
Size Selection of ATAC-seq Libraries
6:48
Results: Quality Control and Next Generation Sequencing Reads
8:07
Conclusion
Trascrizione
The overall goal of this high-throughput method using transposase is to identify accessible chromatin regions in the human genome. This method can help answer key questions in the epigenomic fields such as genome-wide locations of regulatory eleme
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Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.