Enzyme-based Homogenization of cECM Micro-particles
6:56
Results: Effective Removal of Cellular Material Enhances Analysis
8:10
Conclusion
Trascrizione
The overall goal of this procedure is to process cardiac extracellular matrix to a hydrogel, which enables studying the effect of the matrix in various cell based assays. This method can help to answer key questions in the cardiac field, such as h
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This protocol describes the complete decellularization of human myocardium while preserving its extracellular matrix components. Further processing of the extracellular matrix results in the production of microparticles and a cytoprotective self-assembling hydrogel.