Microsampling Single Cells and Metabolite Extraction
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Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) Measurement
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Results: Metabolic Differences Between Single Cells in the Early Developing Xenopus laevis Embryo
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Conclusion
Trascrizione
The overall goal of this protocol is to characterize metabolites in live cells of developing embryos by in situ microsampling capillary electrophoresis-mass spectrometry. This method can help answer key questions in the cell and developmental biol
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We describe steps that enable fast in situ sampling of a small portion of an individual cell with high precision and minimal invasion using capillary-based micro-sampling, to facilitate chemical characterization of a snapshot of metabolic activity in live embryos using a custom-built single cell capillary electrophoresis and mass spectrometry platform.