Melanoma Cell-derived Factor Harvest and Bone Marrow-derived Adipocyte Induction
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Red Oil O Staining and Quantitative Polymerase Chain Reaction (qPCR) Analysis
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Bone Marrow Adipocyte-melanoma Cell Co-culture
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Results: Dynamic Bone Marrow-derived Adipocyte Regulation of Tumor Cells
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Conclusion
Trascrizione
This method can help answer key questions in the bone metastasis research field, such as what changes are induced in the bone marrow adipocyte growth and their development in a cancer microenvironment. The big advantages of this technique are that
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Here, we present a reliable and straightforward two-dimensional (2D) coculture system for studying the interaction between tumor cells and bone marrow adipocytes, which reveals a dual effect of melanoma cell-derived factors on the bone marrow adipocytes differentiation and also poses a classic method for the mechanistic study of bone metastasis.