Tissue Sampling for Ribonucleic Acid (RNA) Extraction and Homogenization
3:22
RNA Extraction from Tissue
5:47
Digestion of Remaining DNA
6:51
Results: LPS Injection Induces the Expression of Pro-inflammatory Cytokines in C57Bl/6 Mice
7:43
Conclusion
Trascrizione
The overall goal of this experiment is to present a model that mimics the entrance of microbial-derived products after an intestinal barrier breach. This model can be used to investigate immune responses after invasion of microorganisms. This meth
Sign in or start your free trial to access this content
Here a protocol to mimic the entrance of bacterial-derived compounds after intestinal barrier breach is presented. A low sublethal dose of lipopolysaccharide was injected systemically into mice, which were monitored for 24 h post-injection. The expression of pro-inflammatory cytokines was determined at several time points in spleen, liver, and colon.