Harvesting Cells Cultured Under Hypoxic Conditions
3:30
Preparing Nuclear Extracts
5:52
Immunoprecipitation of Proteins
7:55
Results: HIF-1α Interacts with ARNT at Hypoxia
8:56
Conclusion
Trascrizione
This method can help answer key questions in the hypoxic field, such as endogenous and native interactions between transcription factors and their transcriptional coregulators under hypoxic conditions. The main advantage of this technique is that
Accedi o inizia la tua prova gratuita per vedere questo contenuto
Here we describe a co-immunoprecipitation protocol to study protein-protein interactions between endogenous nuclear proteins under hypoxic conditions. This method is suitable for demonstration of the interactions between transcription factors and transcriptional co-regulators at hypoxia.