The overall goal of this protocol is to biochemically characterize tRNA-Isopentenyl Transferase Activity In Vitro. This method is useful for the in-vitro detection and characterization of tRNA-isopentenyl transferase activity of purified recombina
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Here, we describe a protocol for the biochemical characterization of the yeast RNA-modifying enzyme, Mod5, and discuss how this protocol could be applied to other RNA-modifying enzymes.