Determining Replicative and Cytotoxic Capacities of Viral Vectors Encoding Immunomodulators
8:03
Assessment of Immunological Functionality of Isolated Virus-encoded Immunomodulators from Infected Cells
10:00
Results: Evaluation of Tumor-targeted Immunomodulation by Paramyxoviruses
11:37
Conclusion
Trascrizione
This method can help address key challenges in the field of cancer immunotherapy by facilitating the development of oncolyctic vectors for targeted immunomodulation. The main advantage of the applied reverse genetic system is its versatility;there
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This protocol describes a detailed workflow for the generation and ex vivo characterization of oncolytic viruses for expression of immunomodulators, using measles viruses encoding bispecific T cell engagers as an example. Application and adaptation to other vector platforms and transgenes will accelerate the development of novel immunovirotherapeutics for clinical translation.