Assay for Transposase-accessible Chromatin Sequencing (ATAC-seq)
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Results: ATAC-seq and Sequencing Result Analysis
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Conclusion
Trascrizione
This modified ATAC-seq protocol introduces a new lysis buffer that decreases contaminating mitochondrial reads from 50%to less than 3%The decreased mitochondrial contamination will allow researchers to perform more efficient ATAC-seq with a 50%dec
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Here, we present a protocol to perform an assay for transposase-accessible chromatin sequencing (ATAC-seq) on activated CD4+ human lymphocytes. The protocol has been modified to minimize contaminating mitochondrial DNA reads from 50% to 3% through the introduction of a new lysis buffer.