Preparation of Chrome Azurol S (CAS)-Fe-Agar Medium
3:35
Pyoverdine/EDTA Standard Preparation
5:04
Collection of Environmental Samples: Soil and Plant Tissues
6:38
Preparation of Siderophore Enrichment Cultures and CAS-Fe Siderophore Production Assay
8:11
CAS-Fe Agar Assays for Detection of Siderophore Production in Environmental Samples
9:34
Results: Pyoverdine Equivalents of Siderophore Enrichment Cultures
11:28
Conclusion
Trascrizione
Siderophores are low molecular weight, metal-chelating biomolecules involved in iron cycling in the environment. This protocol allows for rapid high throughput assessment of siderophore activity in soil and plant samples. Previous methods for side
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We present a protocol for rapid screening of environmental samples for siderophore potential contributing to micronutrient bioavailability and turnover in terrestrial systems.